Determination Of A Bacteriophage Titer

last of a bacteriophage titre Microbiology Lab promulgate II Title: Determination of a Bacteriophage titre Purpose: To determine the human activity of phage particles or plaque-forming units in a breach of T4 bacteriophage. Materials: 18 24 hour line of business culture of Escherichia coli B. 2 ml suspension of T4 bacteriophages with a titre of at least 10,000 phages/ml 5 trypticase soya garret agar (TSA) plates. These should be warm to 37c before use 5 tubes of aristocratic agar (0.7% agar). preliminary to use, melt and hold at 50c in a water supply bath 5 tubes of 9.

0 ml trypticase soy (TS) broth 1 ml sterile pipets pipette support Methods: 1. We began the experiment by marking the plates and place them in lay from 10^5, 10^6, 10^7, then pee-pee the uncontaminating dilutions ready in devote from 10^-4, 10^-5, 10^-6 2. The pipettes was used and pipette 0.5 ml of the phage suspension to the 10^4 3. The adjoining step was pip...If you want to consider a full essay, order it on our website: Orderessay

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